Plant pigment chromatography essay

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Plant pigment chromatography essay

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The process of chromatography separates molecules because of the different solubilities of the molecules in a selected solvent.

The solvent carried the dissolved pigments as it moved up the paper. The pigments were carried at different rates because they were not equally soluble. The most soluble pigment traveled the longest distance while the others traveled in a shorter length. The distance of the pigment traveled was unique for that pigment in set conditions and was used to identify the pigment.

The ratio was then used to measure the Rf retention factor value. Glucose is a simple carbohydrate that provides immediate fuel to cells but it is also a building block for more complex carbohydrates stored by living organisms for future use. For photosynthesis to transform light energy from the sun into chemical energy bond energy in plants, the pigment molecules absorb light to power the chemical reactions.

Plant pigments are macromolecules produced by the plant, and these pigments absorb specified wavelengths of visible light to provide the energy required for photosynthesis.

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Chlorophyll is necessary for photosynthesis, but accessory pigments collect and transfer energy to chlorophyll. Although pigments absorb light, the wavelengths of light that are not absorbed by the plant pigments are reflected back to the eye. The reflected wavelengths are the colors we see in observing the plant.

Plants contain different pigments, and some of the pigments observed include: Flavonoids, anthocyanins, and carotenoids are just some of the categories of plant pigments known to have antioxidant properties.

Published: Thu, 24 May The aim of this experiment was to separate and isolate the different photosynthetic pigments, found on spinach leaves and to extract them using the paper chromatography . Pigments Essay examples - Pigments From prehistoric times, humans had discovered that pigments could be used to colour a surface and they have left their mark on their environment in the form of painted images [7]. Prehistoric painters used the pigments available in the vicinity of their homes. Plants have four types of pigments, namely chlorophyll, carotenoids, anthocyanins, and xanthophylls. These pigments have different polarities and chemical properties. In paper chromatography, the pigments will separate based on their affinity to the medium (paper), and affinity for the solvent.

Many lines of research suggest that consuming a diet rich in plant pigments may slow the process of cellular aging and reduce the risks of some types of disease, such as cancer, heart disease, and stroke.

The point of this experiment is to look at the polarity of some of the common pigments in plant leaves and how that polarity affects their interactions with the cellulose fibers in paper and a few solvents and to apply the technique of paper chromatography as a method for separating individual plant pigments contained in plant tissue extracts containing pigment blends.

These solvents are used because they are capable of separating mixtures that contain both polar and non-polar compounds, or to increase separation of mixtures of compounds that have similar behavior with a single solvent.

As the solvent slowly travels up the paper, the different components of the extract travel at different rates and the extracts are separated into different colors. After minutes, the distance traveled by each pigment and solvent were measured. Pigments and Rf values for each plant extract The distance traveled relative to the solvent is called the Rf value, or the Retardation value.

It can be computed with the formula: It also means that it is less polar because it interacts less strongly with the polar absorbent on the filter paper. So similarly, the smaller the Rf value a compound has, the shorter the distance it travelled. It also means that is is more polar because it interacts more strongly with the polar absorbent on the filter paper.

The distances travelled by the pigments were significantly different than ours because they used different percentages of solvents.

The pigments dissolved in the solvent and migrated upward. We have always understood chlorophyll, a pigment that is very important in photosynthesis, to be green. However, through this experiment we have discovered that many other pigments are also present in the leaves.

For example the kangkong leaf also contains different pigments even though the leaf is dominated by the color green.

Plant pigment chromatography essay

Foundations of Organic Chemistry. Cengage Learning Asia Pte Ltd. Illustrated guide to Home Chemistry experiments.PHYSIOLOGY OF FLOWERING. Plants, to begin with go through a period of vegetative growth. The extent of vegetative growth is endowed with its genetic potentiality.

Published: Thu, 24 May The aim of this experiment was to separate and isolate the different photosynthetic pigments, found on spinach leaves and to extract them using the paper chromatography . Botany: Botany, branch of biology that deals with the study of plants, including their structure, properties, and biochemical processes.

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Also included are plant classification and the study of plant diseases and of interactions with the environment. The principles and findings of botany have provided the.

Separation of Photosynthetic Pigments: Paper Chromatography. Print Reference this an example of which is plant pigments.

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The result of chromatography is a chromatogram. From the chromatogram, different information about the test sample can be attained. If you are the original writer of this essay and no longer wish to have the essay.

In chromatography the solvent moves up a piece of paper by capillary action, which occurs because of the attraction of the solvent molecules to each other and the thin paper.

Chromatography of Pigments - Essay

As the solvents moves up the paper it separates the pigments into its components. The substances that are seen on the paper are plant pigments.


Extraction and Chromatic Separation of Plant Pigments from Tomato Paste; Extraction and Chromatic Separation of Plant Pigments from Tomato Paste. After the two pigments are isolated, column chromatography .

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