Get Full Essay Get access to this section to get all help you need with your essay and educational issues. The daphnia was placed on a slide with cover slip in order to examine its heartbeat by using the microscope. Aim In this Lab we will study the effect of different concentrations of adrenalin on the heart rate daphnia, the water flea. It is expected to recognize a threshold of the daphnia in its reaction the three different adrenalin concentrations, in terms of heart rate.
Hire Writer Figure 1 2 With the low-power objective, locate the transparent heart of the daphnia. It is a beating found almost midway in the back.
Observe the animal for few minutes until it calms down. Figure 2 3 Determine the heartbeat rate over a second period. Your partner should tell you when to start and stop counting.
If the heart is beating too fast count every other beat and multiply by two. Another method of counting is to tap a pencil on a clean sheet of paper each time the heart beats. After 15 seconds, count the number of pencil marks. Record the number of heartbeats per 15 seconds in Table 1.
Multiply this number by 4 to get the number of heartbeats per minute. Record this number in 4 Repeat Step 3 two more times. Trials 2 and 3 b. Record the number of heartbeats per 15 seconds and the number of heartbeats per minute in Table 1.
Average the heartbeats per minute and record this number. Touch a piece of paper towel to the other side of the cover slip to draw the solution through. Add a second drop and draw it through in the same manner.
Wait about 20 seconds. Count the number of heartbeats in 15 seconds. Record your result in Table 1. Trials 5 and 6 d.
Record your results in Table 1. Average the heartbeats per minute and record 7 Repeat Step 5 and 6 above using the 0. Average the heartbeats per minute and record 8 Repeat Steps 5 and 6 above using the 0. Do not return it to the daphnia aquarium. Complete Table 1 by reporting your averages to your teacher for class average determination.
At the beginning the heart rate was still quite high, probably due to the fact, that the daphnia was nervous and scared after being removed from its previous location. Later on in the experiment the heart ate even decreased up to the point where we were using the 0.
After having added the strongest concentration, there must have been some threshold which was over come, because the heart rate rose noticeably from about and to ; this is a clear positive change in heart beat. Questions or Discussion 1. How would you expect adrenalin to affect daphnia? This will only be the case, if its threshold lies at this level.
At what concentration did the adrenalin seem to take effect threshold level?
One could see quite quickly, that the heartbeat rate had increased only b looking at it through the microscope. How do your results compare with the class average?
This might have been due to our impatiens at the beginning of the lab. How much concentrated is 0. Why did you not return your daphnia to the original culture dish aquarium after the activity? This was due to the fact that we had already used these daphnia for our experiment and were therefore contaminated.
If one would have added them back into the aquarium, the other daphnia would have gotten affected due to the other, added daphnia adrenalin. This would have contaminated all the other daphnia and make the further lab procedures impossible to complete. Conclusion As expected, the daphnia threshold could be identified.
This was at 0. How to cite this page Choose cite format:AbstractFor this experiment we tested the effects of different adrenalin concentrations (%; %; %) on the daphnia flea.
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Investigating the effect of chemicals. k Take a large Daphnia from the stock culture and record its heart beat at room temperature in pond water (as in step e).
l Add one drop of 1% ethanol to 5 cm 3 of pond water in a beaker. Mix well. Draw the pond water off the Daphnia with a pipette and replace it with 2 or 3 cm 3 of the water containing ethanol (Note 3). Record the rate of heart beat again. The Effects of Temperature, Acetylcholine, and Adrenaline on the Heart Rate of Daphnia magna Hieu Duong 6 April Introduction The heart is a muscular %(4).